Table 2. Ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS). MS data and concentration of major phenolic compounds found in infusion and fermented products.
| Compound |
tR min |
[M-H]– m/z |
MS/MS ions | γ/(µg/mL) | ||||
|---|---|---|---|---|---|---|---|---|
| Litsea glaucescens | Eucalyptus camaldulensis | |||||||
| Infusion | Fermented beverage | Infusion | Fermented beverage | |||||
| Gallocatechin | 1.19 | 305 | 164, 125 | 2.2±0.2 | 1.78±0.09 | 1.41±0.08 | 2.6±0.3 | |
| Epigallocatechin | 1.85 | 305 | 164, 125 | 6.4±0.3 | 4.58±0.03 | 2.66±0.07 | 5.57±0.09 | |
| Catechin | 1.91 | 289 | 203, 109 | 10.2±0.2 | 5.6±0.9 | 1.6±0.2 | 3.3±0.5 | |
| Gallocatechin gallate | 2.26 | 457 | 305, 169, 125 | 32.7±0.7 | 18.1±2.4 | 14.3±0.8 | 25.3±0.6 | |
| Epicatechin | 2.42 | 289 | 162, 125 | 23.5±0.4 | 14.2±1.4 | 1.3±0.3 | 1.7±0.1 | |
| Epigallocatechin gallate | 2.46 | 457 | 305, 169, 125 | N.D. | N.D. | 1.92±0.03 | 2.7±0.1 | |
| Epicatechin gallate | 2.80 | 441 | 289, 169 | 10.5±0.7 | 6.6±0.7 | 4.22±0.08 | 8.4±0.8 | |
| Total content of analyzed flavan-3-ols | (85.6±1.3)a | (50.9±5.4)b | (27.5±1.3)c | (49.7±1.7)d | ||||
| Rutin | 3.45 | 609 | 301, 271 | 13.7±1.5 | 7.4±0.1 | N.D. | N.D. | |
| Kaempferol | 4.48 | 285 | 187 | 0.3±0.0 | 0.3±0.00 | N.D. | N.D. | |
| Quercetin | 4.62 | 301 | 179, 151 | 10.9±0.5 | 7.5±0.02 | 1.8±0.2 | 3.6±0.4 | |
| Total content of analyzed flavonols | (25.0±1.0)a | (15.3±1.0)b | (1.8±0.2)c | (3.6±0.3)c | ||||
| Total content of analyzed flavonoids | (110.6±2.4)a | (66.2±4.4)b | (29.3±1.6)c | (53.4±1.3)d | ||||
Values are expressed as means±standard error (N=2). Values in a row followed by different letters for each infusion and fermented beverage are significantly different (p<0.05) by Tukey’s test. tR=retention time, N.D.=not detected