Table 2. Identification and quantitiy of proteins and fragments significantly affected by the sampling time (p<0.05).

Spot no.	Protein name	Accession no.	Organism	Location	Mascot score	AAS %	MP	Theorical M/kDa pH	Experimental M/kDa pH	Fold change t/day
										3	7
1	actin, alpha cardiac muscle 1	Q3ZC07	Bos taurus	cytoplasm	315	12	3	42.3 5.23	39 5.66	–1.14	–1.54
25	actin, alpha cardiac muscle 1	Q3ZC07	Bos taurus	cytoplasm	263	12	3	42.3 5 .23	41 5.79	–1.12	–1.29
26	actin, alpha cardiac muscle 1	Q3ZC07	Bos taurus	cytoplasm	264	12	3	42.3 5.23	41 5.94	–1.06	–1.44
36	actin, alpha cardiac muscle 1 (single peptide)	Q0PGG4	Bos taurus	cytoplasm	53	4	1	42.0 5.23	41 7.24	+2.04	+2.54
27	F-actin-capping protein subunit alpha-2	Q3T1K5	Rattus norvegicus	cytoplasm	92	13	3	33.1 5.57	32 5.86	+1.03	+2.27
14	troponin T, slow skeletal muscle	Q75ZZ6	Sus scrofa	cytoplasm	394	26	6	31.2 5.92	30 7.08	–1.06	–1.15
23	troponin T, slow skeletal muscle	Q7TNB2	Rattus norvegicus	cytoplasm	167	9	2	31.2 5.71	34 5.97	–1.71	–2.25
35	troponin T, fast skeletal muscle (single peptide)	Q8MKI3	Bos taurus	cytoplasm	80	5	1	32.1 5.99	37 7.11	+1.29	+1.37
17	heat shock protein beta-6 (single peptide) HSP20 (17 kDA)	Q148F8	Bos taurus	cytoplasm	67	6	1	17.5 5.95	17 6.38	+1.42	+1.78
28	heat shock protein beta-1 (single peptide) HSP27 (17 kDA)	Q3T149	Bos taurus	cytoplasm	122	7	1	22.4 5.98	22 5.98	–1.32	+1.15
53	NADH dehydrogenase [ubi- quinone] iron-sulfur protein 3	Q0MQG6	Pongo pygmaeus	mito- chondria	133	9	2	30.4 6.54	26 5.78	–1.06	–1.39
62	desmin (single peptide)	Q5XFN2	Canis familiaris	cytoplasm	49	3	1	53.3 5.21	51 5.19	+1.07	+1.96
3	unidentified protein
45	unidentified protein

Protein names and accession numbers were derived from the UniProt database; AAS=coverage of the entire amino acid sequence, MP=number of matched peptides, theorical=recorded in UniProt database and experimental=calculated from the spot position on the gel