Table 1. Effect of surfactants and organic solvents on mycelial growth and exopolysaccharide (EPS) production by Lentinus tigrinus in shake flask cultures.
| γ(mycelium)/(g/L) | γ(EPS)/(g/L) | |
|---|---|---|
| Control | 9.47±0.05 | 3.35±0.09 |
| Surfactant | ||
| Tween 40 | 10.40±0.12 | 3.07±0.06 |
| Tween 60 | 4.80±0.01 | 2.38±0.09 |
| Tween 80 | 5.70±0.02 | 4.10±0.27 |
| Organic solvent | ||
| Chloroform | 13.40±0.03 | 5.15±0.74 |
| Ethanol | 8.67±0.02 | 5.36±0.11 |
| DMSO | 8.30±0.01 | 3.29±0.38 |
| Acetone | 7.80±0.01 | 6.94±0.09 |
Fermentations were carried out for 12 days at 26 °C. The surfactants and organic solvents were added to the basal medium at a volume fraction of 0.2% after 8 days of cultivation. Values are mean±S.D. of triplicate determinations