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. 2004 Sep;186(18):6265–6276. doi: 10.1128/JB.186.18.6265-6276.2004

FIG. 3.

FIG. 3.

Western analysis of PrfA protein levels produced by the various L. monocytogenes strains. Soluble bacterial whole-cell lysates were prepared from mid-log-phase cultures. The strain numbers and relevant genotypes are shown above the lanes. PrfA was detected by using a monoclonal antibody against PrfA and an alkaline phosphatase-conjugated goat anti-mouse secondary antibody. (A) PrfA protein levels from L. monocytogenes integrant strains in the NF-L1003 (ΔprfA) background. Equal amounts of total protein (23 μg) solubilized in SDS-PAGE sample buffer were loaded for each sample. (B) Quantitative comparison of PrfA protein levels from WT strain NF-L476 (476) and original EMS mutant strain NF-L879 (879), with the amount of total proteins loaded in each lane indicated. (C) Quantitative comparison of PrfA protein levels from integrant strains NF-L1041 (ΔprfA + WTi) (1041) and NF-L1011 (ΔprfA + L140Fi) (1011), with the amount of total proteins loaded in each lane indicated.