TABLE 1.
Bacterial strains used in this study
| Strain | Relevant descriptiona | Source or reference |
|---|---|---|
| DH5α | E. coli strain for constructing recombinant plasmids | |
| SM10 | E. coli strain as conjugation donor for pPL2 plasmid or constructs | 44 |
| NF-L476 | L. monocytogenes WT strain 10403S with actA-gus-plcB transcriptional fusion | 42 |
| NF-L758 | NF-L476 with an erythromycin resistance gene (erm) inserted downstream of orfXYZ at the 3′ end of the PrfA regulon | 43 |
| NF-L879 | NF-L476 EMS mutant with increased level of in vitro actA expression | 43 |
| NF-L1003 | NF-L476 ΔprfA with erythromycin resistance gene inserted downstream of the PrfA regulon | This work |
| NF-L1009 | NF-L1003 tRNAArg::pPL2 (ΔprfA + pPL2i) | This work |
| NF-L1041 | NF-L1003 tRNAArg::pPL2-prfA476 (ΔprfA + WTi) | This work |
| NF-L1042 | NF-L1003 tRNAArg::pPL2-prfAL147P (ΔprfA + L147Pi) | This work |
| NF-L1011 | NF-L1003 tRNAArg::pPL2-prfAL140F (ΔprfA + L140Fi) | This work |
| NF-L1006 | NF-L476 tRNAArg::pPL2 (WT + pPL2i) | This work |
| NF-L1039 | NF-L476 tRNAArg::pPL2-prfA476 (WT + WTi) | This work |
| NF-L1040 | NF-L476 tRNAArg::pPL2-prfAL147P (WT + L147Pi) | This work |
| NF-L1008 | NF-L476 tRNAArg::pPL2-prfAL140F (WT + L140Fi) | This work |
| NF-L1067 | NF-L879 tRNAArg::pPL2-prfAL140F (L140F + L140Fi) | This work |
a
pPL2i is the integration of pPL2 at tRNAArg-attBB′ of the chromosome. i indicates the integration of the particular prfA allele at tRNAArg-attBB′ of the chromosome.