TABLE 2.

Phenotypic analysis of L. monocytogenes strains with prfA mutations

Strain	PrfA genotype	Hemolytic activitya	Plaque sizeb	Motilityc
NF-L476	WT	100	100d	100
NF-L879	L140F	461.5 ± 225.3	78.9 ± 2.7d	0.8 ± 28.5
NF-L1003	ΔprfA	20.8 ± 22.7	0	114.2 ± 14.1
NF-L1009	ΔprfA + pPL2i	16.3 ± 19.1	0	121.4 ± 14.7
NF-L1041	ΔprfA + WTi	75.6 ± 28.4	100e	148.6 ± 24.0
NF-L1042	ΔprfA + L147Pi	24.5 ± 10.3	0	145.1 ± 28.7
NF-L1011	ΔprfA + L140Fi	564.0 ± 164.8	90.4 ± 2.2e	46.4 ± 22.5
NF-L1006	WT + pPL2i	86.2 ± 27.6	100f	85.6 ± 11.8
NF-L1039	WT + WTi	187.7 ± 54.4	109.1 ± 4.3f	111.0 ± 13.4
NF-L1040	WT + L147Pi	82.2 ± 20.7	107.0 ± 5.4f	130.1 ± 22.8
NF-L1008	WT + L140Fi	884.1 ± 598.7	104.1 ± 8.9f	7.2 ± 26.7
NF-L1067	L140F + L140Fi	312.9 ± 35.9	100.6 ± 4.9e	4.3 ± 21.1

^aDetermined after 5 h of growth in BHI broth at 37°C; expressed as percentage ± standard deviation from at least three independent experiments, with WT set at 100%.

^bExpressed as percentage ± standard deviation from four independent experiments.

^cSoft agar (BHI-activated charcoal with 0.3% [wt/vol] agar) was inoculated with mid-log-phase bacterial cultures and incubated overnight at 37°C. Motility was measured as the diameter of the swimming colony minus that of a ΔflaA mutant and is expressed as percentage ± standard deviation from duplicate samples from two experiments, with WT set at 100%.

^dPercent ± standard deviation with WT (NF-L476) set at 100%.

^ePercent ± standard deviation with NF-L1041 set at 100%.

^fPercent ± standard deviation with NF-L1006 set at 100%.