FIG. 2.

The F0/F1 proton-translocating ATPase is required by AR1. (A) Cells (EK227 and EF996) were grown in LB MOPS (pH 8.0) or LB MES (pH 5.5) to stationary phase and diluted 1:1,000 into EG pH 2.5 medium without exogenous amino acids (pH 5.5 induces AR1 function). (B) The same cells were grown in LBG to repress AR1 and diluted 1:1,000 into EG pH 2.5 medium, with or without 1.6 mM sodium glutamate. (C) Cells were grown in BHIG to repress AR1 and diluted 1:1,000 into EG pH 2.5 medium with or without 1 mM l-arginine. *, below the level of detection.