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Deletion of the PD-3 promoter does not affect hag gene (A) and fla-che operon (B) expression. hag-lacZ (9) and ylxF-lacZ (Fig. 1C) transcriptional fusions were used to monitor the expression of the flagellin hag gene and the fla-che operon, respectively. The strains were PB5128 and BFA2666 (wild type; filled circles), PB5324 and PB5328 (ΔPD-3; open circles), PB5267 and PB5279 [degU32(Hy); open squares], and PB5325 and PB5329 [degU32(Hy) ΔPD-3; filled squares].
