TABLE 1.
Bacterial strains and plasmids used in this work
| Strains and plasmids | Descriptiona | Source or referenceb |
|---|---|---|
| B. subtilis strains | ||
| 1A1 | trpC2 | BGSCc |
| LU60A1 | trpC2 ΔccdA::ble; Pmr | 32 |
| LUA13 | trpC2 ΩcotC-lacZ; Cmr | 31 |
| LUA14 | trpC2 ΔspoIIIG::neo; Nmr | 31 |
| LUL3 | trpC2 bdbCΩpLLE21; Emr | 10 |
| LUL9 | trpC2 resAΩpLLE36; Emr | 9 |
| LUL10 | trpC2 bdbDΩTn10; Spr | 10 |
| LUL20 | trpC2 stoAΩpLLE39; Cmr | This work; pLLE39 → 1A1 |
| LUL21 | trpC2 stoAΩpLLE39 ΔccdA::ble; Cmr Pmr | This work; LUL20 → LU60A1 |
| LUL23 | trpC2 stoAΩpLLE39 bdbDΩTn10; Cmr Spr | This work; LUL10 → LUL20 |
| LUL24 | trpC2 stoAΩpLLE39 Δ(bdbA-yolJ-bdbB)::kan; Cmr Kmr | This work; LUL110 → LUL20 |
| LUL25 | trpC2 stoAΩpLLE39 ΔyneN::erm; Cmr Emr | This work; LUL121 → LUL20 |
| LUL27 | trpC2 bdbDΩTn10 Δ(bdbA-yolJ-bdbB)::kan; Spr Kmr | This work; LUL10 → LUL110 |
| LUL30 | trpC2 Δ(ykvU-stoA)::tet; Tcr | This work; pLLE77 → 1A1 |
| LUL35 | trpC2 ΩcotC-lacZ; Spr | This work; pCm::Sp → LUA13 |
| LUL36 | trpC2 stoAΩpLLE39 ΩcotC-lacZ; Spr | This work; LUL35 → LUL20 |
| LUL110 | trpC2 Δ(bdbA-yolJ-bdbB)::kan; Kmr | This work; pLLE9 → 1A1 |
| LUL121 | trpC2 ΔyneN::erm; Emr | This work; pLLE16 → 1A1 |
| Plasmids | ||
| pBAD-HisA/B | Expression vectors for His-tagged proteins; Amr | In vitrogen |
| pDG148 | Expression vector; Emr Kmr | 35 |
| pDG647 | Carries antibiotic resistance cassette; Emr Amr | 13 |
| pDG780 | Carries antibiotic resistance cassette; Kmr Amr | 13 |
| pDG1515 | Carries antibiotic resistance cassette; Tcr Amr | 13 |
| pHV32 | Integration vector for B. subtilis; Cmr Tcr Amr | 25 |
| pPHO1 | pET21-(+) with a 1.4-kb SacI-HindIII fragment containing truncated E. coli phoA from pPhoA; Amr | Mimmi Throne-Holst and reference 28 |
| pRAN1 | pHPSK with a 0.73-kb fragment containing B. subtilis resA; Cmr Emr | 9 |
| pCm::Sp | Plasmid for changing antibiotic resistance; Spr | 34 |
| pLLE9 | pDG780 with B. subtilis chromosomal sequences flanking the kanamycin resistance gene; Kmr Amr | This work |
| pLLE16 | pDG647 with B. subtilis chromosomal sequences flanking the erythromycin resistance gene; Emr Amr | This work |
| pLLE34 | pBAD-HisA with a 609-bp fragment containing a truncated bdbD gene; Amr | This work |
| pLLE39 | 310-bp internal fragment from stoA in pHV32; Cmr Tcr Amr | This work |
| pLLE64 | pPHO1 with a 271-bp fragment containing the 5′ part of stoA; Amr | This work |
| pLLE65 | pBAD-HisB with a 443-bp fragment containing a truncated stoA gene; Amr | This work |
| pLLE77 | pDG1515 with B. subtilis chromosomal sequences flanking the tetracycline gene; Tcr Amr | This work |
| pLLE82 | pDG148 with a 0.73-kb HindIII-ScaI fragment containing B. subtilis resA from pRAN1; Emr Kmr | This work |
| pLLE83 | pDG148 with a 537-bp fragment containing the stoA gene; Emr Kmr | This work |
a
Amr, Cmr, Emr, Kmr, Nmr, Spr, Pmr, and Tcr indicate resistance to ampicillin, chloramphenicol, erythromycin, kanamycin, neomycin, spectinomycin, phleomycin, and tetracycline, respectively.
b
An arrow indicates transformation of the indicated strain with chromosomal or plasmid DNA.
c
Bacillus Genetic Stock Center, Columbus, Ohio.