TABLE 3.
Sporulation efficiency of B. subtilis strainsa
| Strain | Relevant genotype | Total cell titerb | Spore titerc | Sporulation efficiency (%)d |
|---|---|---|---|---|
| 1A1 | Wild type | 6.5 × 108 | 6.4 × 108 | 98 |
| LU60A1 | ΔccdA::ble | 8.7 × 107 | 3.9 × 106 | 4 |
| LUL20 | stoAΩpLLE39 | 7.0 × 107 | 3.3 × 104 | 0.05 |
| LUL121 | ΔyneN::erm | 5.0 × 108 | 5.0 × 108 | 100 |
| LUL21 | stoAΩpLLE39 ΔccdA::ble | 9.7 × 107 | 5.6 × 103 | 0.006 |
| LUL25 | stoAΩpLLE39 ΔyneN::erm | 1.1 × 108 | 4.9 × 104 | 0.04 |
| LUL30 | Δ(ykvU-stoA)::tet | 3.7 × 108 | 3.2 × 102 | 0.0001 |
| LUL3 | bdbCΩpLLE21 | 5.1 × 108 | 4.9 × 108 | 96 |
| LUL10 | bdbDΩTn10 | 5.2 × 108 | 4.9 × 108 | 94 |
| LUL110 | Δ(bdbA-yolJ-bdbB)::kan | 5.3 × 108 | 4.8 × 108 | 90 |
| LUL23 | stoAΩpLLE39 bdbDΩTn10 | 6.0 × 108 | 5.9 × 108 | 98 |
| LUL24 | stoAΩpLLE39 Δ(bdbA-yolJ-bdbB)::kan | 8.8 × 107 | 5.4 × 104 | 0.06 |
| LUL27 | bdbDΩTn10 Δ(bdbA-yolJ-bdbB)::kan | 5.7 × 108 | 4.8 × 108 | 84 |
a
In all sporulation assays 1A1 and LU60A1 were included as controls. Experimental results were considered reliable if the numbers for 1A1 and LU60A1 did not deviate more than 20% from previously obtained (10, 31) mean values.
b
Titer is CFU per milliliter.
c
Titer after heat treatment for 15 min at 80°C.
d
Sporulation efficiency is calculated as the spore titer divided by the total cell titer.