TABLE 5.
Complementation of StoA deficiency in strains LUL20 and LUL30 by IPTG-dependent expression of StoA from plasmid pLLE83 (a derivative of vector pDG148)
| Strain | [IPTG]a | Total cell titer | Spore titerb | Sporulation efficiency (%) |
|---|---|---|---|---|
| 1A1/pDG148 | 0 | 8.0 × 108 | 6.6 × 108 | 84 |
| 1A1/pDG148 | 1 | 7.0 × 108 | 5.9 × 108 | 84 |
| 1A1/pLLE83 | 1 | 7.0 × 108 | 7.1 × 108 | 100 |
| LUL20/pDG148 | 0 | 1.6 × 108 | 1 × 105 | 0.06 |
| LUL20/pDG148 | 1 | 1.0 × 108 | 5.9 × 104 | 0.06 |
| LUL20/pLLE83 | 0 | 1.7 × 108 | 2.7 × 106 | 2 |
| LUL20/pLLE83 | 1 | 2.1 × 108 | 1.7 × 108 | 79 |
| LUL30/pDG148 | 1 | 1.6 × 108 | 1.2 × 104 | 0.008 |
| LUL30/pLLE83 | 1 | 2.4 × 108 | 5.0 × 107 | 20 |
a
IPTG concentration (mM) in the growth medium.
b
Assessed by heat treatment (see Table 3).