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Choline uptake activity in S. meliloti wild-type strain Rm5000 and the choX M1A mutant. Uptake of [methyl-14C]choline (10 μM) was assayed in cells grown until the stationary phase in MCAA medium supplemented or not supplemented with 7 mM choline (cho) and maintained at low osmolarity (no salt) or high osmolarity (0.3 M NaCl). The values are means for triplicate experiments from four independent cultures, and the error bars indicate standard deviations.
