Escherichia coli strains |
|
|
DH5α |
FsupE44 ΔlacU169(φ80dlacZΔM15) hsdR17(rK− mK+) recA1 endA1 gyrA96 thi-I relA1
|
Bethesda Research Laboratories |
MT616 |
MT607(pRK600) |
8 |
BL21(DE3)(pLysS) |
F ompT hasdSB (rB− mB−) gal dcm (DE3) pLysS (Cmr) |
Novagen |
Sinorhizobium meliloti strains |
|
|
RCR2011 |
SU47, wild type |
30 |
1021 |
Derivative of RCR2011, Strr
|
20 |
GM1211 |
Derivative of RCR2011, Strr Lac |
23 |
5000 |
Derivative or RCR2011, Rifr
|
8 |
M1A |
Rm5000 choX::Ω, Rifr Spr
|
This study |
Plasmids |
|
|
PLAFR3 |
IncP cosmid cloning vector, Tcr
|
10 |
pRK600 |
ColE1 replicon with RK2 transfer region, Cmr
|
8 |
pBluescriptSK(−) |
Derivative of pUC19 with f1(−)oriR, Ampr
|
Stratagene |
pGEM T-3Zf(+) |
Cloning vector |
Promega |
pET20-b(+) |
Derivative of pBR322, T7 promoter, 3′ His codons, Ampr
|
Novagen |
pSUP202 |
ColE1, Mob+ Tcr Ampr Cmr
|
33 |
pHP45-Ω |
Apr, pBR322 derivative with interposon Ω Smr/Spr
|
28 |
pGQ5 |
pGEMT vector with bup1-pro4-amplified fragment |
This study |
pF1 |
pLAFR3 with an S. meliloti 16-kb insert containing cho locus |
This study |
p1.2E |
1.2-kb EcoRI fragment from pF1 cloned into pBSSK− vector |
This study |
p1.5E |
1.5-kb EcoRI fragment from pF1 cloned into pBSSK− vector |
This study |
p3.4HE |
3.4-kb HindIII-EcoRI fragment from pF1 cloned into pBSSK vector |
This study |
p6.5H |
6.5-kb HindIII fragment from pF1 cloned into pBSSK vector |
This study |
pSUP6.5H |
6.5-kb HindIII fragment from pF1 cloned into pSUP202 vector |
This study |
pXΩ |
pSUP6.5H, choX::Ω (BglII insertion) |
This study |
pETNE |
pET20b, Nde1-EcoRI fragment (choX without His codon fusion) |
This study |
pETNX |
pET20b, Nde1-Xho1 fragment (choX with 3′ His codon fusion) |
This study |