TABLE 2.

Oligonucleotide primers used in this study

Primer name	Sequence (5′→3′)a	Descriptionb
CAN 122	GGCCACCGCGACCTGCTGC	Reverse primer hrdB RT-PCR
CAN 123	CGGCCAAGCGCACCACTACC	Forward primer hrdB RT-PCR
ceaS1-S1-For	TCATGAATTCCGGTGGACGGAAGGGGACGG	Forward primer for ceaS1 S1 probe
ceaS1-PR-EX	ATGGCTTTCGCGGTCGTGGT	Reverse primer for ceaS1 S1 probe and primer extension
ceaS2-S1-For	TGGATCCGTCGCGAATCCAGGGAAGCCGAGC	Forward primer for ceaS2 S1 probe
ceaS2-PR-EX	GGGCGGTCGATACACGGG	Reverse primer for ceaS2 S1 probe and primer extension
bls1-S1-For	TCGGATTAATACCTCGCTGCTCGCCGCCCTCAC	Forward primer for bls1 S1 probe
bls1-S1-Rev	GGTCGGGGCCGGGCATGGTGAA	Reverse primer for bls1 S1 probe
pah1-S1-For	CCAGATTAATGCGGCGCGGACGGTGCAG	Forward primer for pah1 S1 and Northern probe
pah1-S1-Rev	CGGGGAGACGGCGGTGGACA	Reverse primer for pah1 S1 and Northern probe
pah1-UP-Rev	CGCGGCTGCCCCTCCCTC	Reverse primer for pah1 S1 probe
ceaS1-RT-For	GCGCAGTCCGAGTCGTAC	Forward primer ceaS1 RT-PCR
ceaS1-RT-Rev	TTGGCGGTGTAGGTGGTGAC	Reverse primer ceaS1 RT-PCR
ceaS2-RT-For	AGGCCGCGTCGATTCTCTTC	Forward primer ceaS2 RT-PCR
ceaS2-RT-Rev	CGGCGGGTTGGGGACGGT	Reverse primer ceaS2 RT-PCR
KTA-ceaS1-For	GCGGGATCCGGGCGGTCAGCACGGT	Forward primer for cloning ceaS1 promoter
KTA-ceaS1-Rev	CCGGGTACCAGGGTCGCGAAGCACG	Reverse primer for cloning ceaS1 promoter
KTA-ceaS2-For	AACCCCAGGATCCGAGCCCCACCGTCACG	Forward primer for cloning ceaS2 promoter
KTA-ceaS2-Rev	CGGCCGGGTACCCCAAACACCTTCCCCACAC	Reverse primer for cloning ceaS2 promoter

^aNonhomologous sequences incorporated into oligonucleotide primers are underlined, and engineered restriction sites are shown in boldface letters.

^bDetails of the function of each oligonucleotide primer are provided in Materials and Methods.