FIG. 2.

Schematic representation of the mca inactivation in M. smegmatis and Southern analysis of mutants versus the parent strain, mc²155. (A) Site of disruption of the Ami37 mca mutant. (B) Southern analysis of BglII-digested genomic DNA from mc²155 (lane 3) and the mutants Ami37 (lane 2) and Ami40 (lane 1). Ten micrograms of DNA was loaded on the gel. The gel was hybridized with a probe for M. smegmatis mca that was PCR DIG-labeled using primers Pgint1082I-5′ and Pgint1082I-3′. The probe hybridized to a 6-kb BglII fragment in the parent strain. The disruption of mca in the mutants introduced a BglII site into the fragment which resulted in the probe hybridizing to two bands of 2 and 5 kb.