TABLE 2.
Amount of substrate MS-mB and product AcCyS-mB of Mca present intracellularly and extracellularly in the parent strain and the mca mutant Ami37 following reaction with mBBr
| Substrate | Amt of substrate for strain:
|
|
|---|---|---|
| mc2155 | Ami37 | |
| Intracellular | ||
| MS-mB (μmol/g) | 2.8 ± 0.02 | 16.6 ± 0.5 |
| AcCyS-mB (μmol/g) | 9.9 ± 0.2 | <0.02 |
| Total | 12.7 | 16.6 ± 0.5 |
| Extracellular | ||
| AcCyS-mB (μM) | 0.75 ± 0.04 | <0.1 |