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. 2004 Sep;186(18):6239–6247. doi: 10.1128/JB.186.18.6239-6247.2004

FIG. 4.

FIG. 4.

Immunodetection of HpaG mutant proteins. Production of the mutant proteins was induced with IPTG induction. After boiling and purification procedures, the proteins were analyzed by immunoblotting with a rabbit polyclonal anti-HpaG antibody. (A) Immunodetection of full-length HpaG mutants. Lanes: 1, pET14b vector control; 2, HpaG; 3, HpaG(L39A); 4, HpaG(L39P); 5, HpaG(L42D); 6, HpaG(L43P); 7, HpaG(Q45A); 8, HpaG(L46A); 9, HpaG(L50A); 10, HpaG(L50P); 11, HpaG(L43P/L50P). (B) Immunodetection of HpaG deletion mutants. Lanes: 1, pET14b vector control; 2, HpaGΔC66; 3, HpaGΔC75; 4, HpaGΔC77; 5, HpaGΔC79; 6, HpaGΔC81; 7, HpaGΔC83; 8, HpaGΔN67.