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Comparison of the HR elicitor activity in tobacco leaves of the XopA mutant proteins and the XopA-HpaG swap protein. The proteins were injected into tobacco leaves at concentrations of 10, 5, 2.5, 1, or 0.5 μM in 20 mM Tris-HCl (pH 8.0). Labeling: 1, HpaG; 2, XopA; 3, XopA(F48L); 4, XopA(M52L); 5, XopA(F48L/M52L); 6, XopA-HpaG swap protein; buffer, 20 mM Tris-HCl (pH 8.0). Tobacco (N. tabacum cv. Samsun NN) leaves were photographed 24 h after injection.
