TABLE 3.
Oligonucleotides used for site-directed mutagenesisa
| Oligonucleotide | Sequence; description |
|---|---|
| M13-20 | 5′-GTAAAACGACGGCCAGTG-3′ |
| hpaGL4A | 5′-ATGAATTCTGCGAACACACAGCTCG-3′; mutagenic primer used to construct HpaG(L4A) |
| hpaGQ7A | 5′-CTTTGAACACAGCGCTCGGCGC-3′; mutagenic primer used to construct HpaG(Q7A) |
| hpaGG9A | 5′-CACAGCTCGCCGCCAACTCGTC-3′; mutagenic primer used to construct HpaG(G9A) |
| hpaGF14D | 5′-CCAACTCGTCCGACTTTCAGGTTGAC-3′; mutagenic primer used to construct HpaG(F14D) |
| hpaGQ16A | 5′-TCCTTCTTTGCGGTTGACCCCG-3′; mutagenic primer used to construct HpaG(Q16A) |
| hpaGP19A | 5′-CAGGTTGACGCCGGCCAGAAC-3′; mutagenic primer used to construct HpaG(P19A) |
| hpaGG20E | 5′-GTTGACCCCGAACAGAACACGC-3′; mutagenic primer used to construct HpaG(G20E) |
| hpaGQ21A | 5′-GACCCCGGCGCGAACACGC-3′; mutagenic primer used to construct HpaG(Q21A) |
| hpaGQ24A | 5′-CCAGAACACGGCATCTAGTCCGAAC-3′; mutagenic primer used to construct HpaG(Q24A) |
| hpaGQ29A | 5′-AGTCCGAACGCGGGCAACCAG-3′; mutagenic primer used to construct HpaG(Q29A) |
| hpaGG30E | 5′-CCGAACCAGGAAAACCAGGGCATC-3′; mutagenic primer used to construct HpaG(G30E) |
| hpaGQ32A | 5′-CAGGGCAACGCGGGCATCTC-3′; mutagenic primer used to construct HpaG(Q32A) |
| hpaGG33A | 5′-CAGGGCAACCAGGCCATCTCGG-3′; mutagenic primer used to construct HpaG(G33A) |
| hpaGL39P | 5′-TCGGAAAAGCAACCGGACCAGC-3′; mutagenic primer used to construct HpaG(L39P) |
| hpaGL42D | 5′-AACTGGACCAGGACCTGACCCAGCTCATC-3′; mutagenic primer used to construct HpaG(L42D) |
| hpaGL50P | 5′-CAGCTCATCATGGCACCGCTGCAGCAG-3′; mutagenic primer used to construct HpaG(L50P) |
| hpaGD99A | 5′-CGTCGGAGCCATTCTCCAG-3′; mutagenic primer used to construct HpaG(D99A) |
| xopAF48L | 5′-GACCCAGCTCATCTTTTCAATG-3′; mutagenic primer used to construct XopA(F48L) |
| xopAM52L | 5′-CTTTTCACTGCTTCTGCAGG-3′; mutagenic primer used to construct XopA(M52L) |
a
Base differences from the wild-type sequence are shown in boldface italic.