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Plant tissue watersoaking assay. (A) Pierced onion bulb scale inoculated with B. cenocepacia strain K56-2 showing partial tissue collapse and translucence characteristic of the ptw phenotype. (B) Pierced onion bulb scale inoculated with sterile water (ptw negative). (C) Pierced onion bulb scale inoculated with ptw-negative mutant AE307. All ptw-negative mutants expressed no ptw activity on onion tissue, and inoculated scales showed the same symptoms as that in panel C.
