TABLE 1.
Plasmids used in this study
| Plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| pK56-2 | 92-kb resident plasmid of B. cenocepacia strain K56-2 containing the ptw cluster | This study |
| pDrive | Cloning vector; Kmr, Apr, pUC origin, T7, SP6, lacZaα+ | Qiagen |
| pRK2013 | Kmr, Tra+, Mob+, ColE1 replicon | 20 |
| pBR325 | Tcr, Apr, Cmr, ColE1 replicon | 4 |
| pR388 | IncW, Sur, Tpr | 13 |
| pTnMod-RTp′ | Tn5, R6K oriR, Tpr | 15 |
| pScosBC1 | Cosmid with K56-2 genomic library | 62 |
| pSBC-9F5 | pScosBC1 with K56-2 genomic DNA containing ptwD4 and ptwC | This study |
| pSBC-3H3 | pScosBC1 with K56-2 genomic DNA containing ptwB4 and ptwB10 | This study |
| pJQ200SK | GmrsacB Mob+, P15A replicon | 55 |
| pURF047 | IncW, Ampr, Gmr, Mob+, lacZα+, Par+, derivative of pURF043 | 63 |
| pASE101 | pURF047 with 2.0-kb Tpr cassette inserted at ScaI site of Apr cassette | This study |
| pASE102 | pJQ200SK containing 10-kb BamHI fragment (pSBC-9F5) containing ptwD4 and ptwC inserted in the MCS | This study |
| pASE103 | pJQ200SK containing 11-kb BamHI fragment (pSBC-3H3) containing ptwB4 and ptwB10 inserted in the MCS | This study |
| pASE104 | pASE101 containing 10-kb BamHI fragment (pSBC-9F5) containing ptwD4 and ptwC inserted in the MCS | This study |
| pASE105 | pASE101 containing 11-kb BamHI fragment (pSBC1-3H3) containing ptwB4 and ptwB10 inserted in the MCS | This study |
| pASE106 | pASE102 with 1.9-kb Tcr cassette from pBR325 inserted in the ScaI site of ptwD4 | This study |
| pASE107 | pASE102 with 1.9-kb Tcr cassette from pBR325 inserted in the EcoRV site of ptwC | This study |
| pASE108 | pASE103 with 1.9-kb Tcr cassette from pBR325 inserted in the ApaI site of ptwB4 | This study |
| pASE109 | pASE103 with 1.9-kb Tcr cassette from pBR325 inserted in the SacI site of ptwB10 | This study |
| pASE110 | pDrive containing 1.5-kb PCR product containing virB6 | This study |
| pASE111 | pDrive containing 1.9-kb PCR product containing virB11 | This study |
| pASE112 | pJQ200SK containing virB6 from pASE110 | This study |
| pASE113 | pJQ200SK containing virB11 from pASE111 | This study |
| pASE114 | pASE112 with 1.9-kb Tcr cassette from pBR325 inserted into the ScaI site of virB6 | This study |
| pASE115 | pASE113 with 1.9 kb Tcr cassette from pBR325 inserted into the ScaI site of virB11 | This study |
a
Ap, ampicillin; Cm, chloramphenicol; Km, kanamyacin; Su, sulphonamide; Tc, tetracycline; Tp, trimethoprim; ptw, plant tissue watersoaking phenotype.