TABLE 2.
Bacterial strains used in this study
| Strain | Relevant characteristicsa | Reference or source |
|---|---|---|
| B. cenocepacia | ||
| K56-2 | CF respritory isolate, ptw+ | 62 |
| GM237 | K56-2 ptwD4::RTp′, ptw− | This study |
| GM238 | K56-2::RTp′, ptw+ | This study |
| GM241 | K56-2::RTp′, ptw− | This study |
| GM242 | K56-2 ptwD4::RTp′, ptw− | This study |
| GM243 | K56-2::RTp′, ptw− | This study |
| AE307 | K56-2 ptwD4::Tc, ptw− | This study |
| AE320 | K56-2 ptwC::Tc, ptw+ | This study |
| AE321 | K56-2 ptwB4::Tc, ptw− | This study |
| AE322 | K56-2 ptwB10::Tc, ptw− | This study |
| AE310 | AE307 (pASE104), ptw+ | This study |
| AE323 | AE321 (pASE105), ptw+ | This study |
| AE324 | AE322 (pASE105), ptw+ | This study |
| AE360 | K56-2 virB6::Tc, ptw+ | This study |
| AE361 | K56-2 virB11::Tc, ptw+ | This study |
| E. coli | ||
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu) 7697 galU galK1 rpsL nupG | Life Technologies, Inc. (Gaithersburg, Md.) |
| HB101 | F−recA13 | 6 |
| CC118λpir | ΔlacX74 galE galK thi-1 rpsE rpoB recA1, lysogenized with λ pir phage | 27 |
a
Tc, tetracycline; ptw, plant tissue watersoaking phenotype.