TABLE 5.
Effect of supernatant concentrates from K56-2, ptwD4 mutant (AE307), and complemented ptwD4 mutant (AE310) on carrot protoplasts
| Supernatant concentrate | Concentrate dilutiona | % Plasmolysisb,c at time point (h):
|
|||
|---|---|---|---|---|---|
| 2 | 4 | 6 | 8 | ||
| M9 medium | 0 | 1.2 ± 1.1x | 2.7 ± 1.6x | 6.6 ± 1.7x | 8.2 ± 3.4x |
| 1:1 | 1.5 ± 1.7x | 3.4 ± 1.2x | 6.4 ± 2.0x | 7.5 ± 2.8x | |
| 1:5 | 2.6 ± 1.0x | 3.1 ± 1.6x | 4.3 ± 1.4x | 6.9 ± 2.7x | |
| 1:10 | 1.9 ± 1.5x | 2.9 ± 1.0x | 5.6 ± 2.3x | 7.3 ± 2.6x | |
| K56-2 | 0d | 18 ± 4.0y | 37 ± 4.1y | 57 ± 5.1y | 75 ± 5.8y |
| 1:1 | 18 ± 2.1y | 38 ± 5.0y | 56 ± 6.1y | 76 ± 7.6y | |
| 1:5 | 18 ± 4.3y | 36 ± 5.0y | 55 ± 5.6y | 73 ± 6.4y | |
| 1:10 | 1.6 ± 1.4x | 3.6 ± 1.0x | 5.9 ± 0.6x | 7.3 ± 1.4x | |
| AE307 | 0d | 1.8 ± 1.2x | 3.3 ± 0.7x | 4.9 ± 1.7x | 6.7 ± 1.7x |
| 1:1 | 2.2 ± 1.3x | 3.8 ± 2.1x | 5.4 ± 1.0x | 7.4 ± 1.9x | |
| 1:5 | 2.3 ± 1.4x | 3.5 ± 0.7x | 5.8 ± 1.5x | 8.4 ± 1.4x | |
| 1:10 | 2.3 ± 1.5x | 4.5 ± 1.1x | 6.1 ± 0.8x | 7.7 ± 0.6x | |
| AE310 | 0d | 19 ± 1.0y | 37 ± 3.0y | 52 ± 4.0y | 78 ± 7.7y |
| 1:1 | 19 ± 1.4y | 36 ± 4.9y | 52 ± 3.7y | 70 ± 6.6y | |
| 1:5 | 18 ± 2.0y | 39 ± 4.5y | 56 ± 4.2y | 77 ± 7.0y | |
| 1:10 | 1.5 ± 1.0x | 1.8 ± 1.0x | 6.0 ± 1.6x | 7.2 ± 1.0x | |
| None | 0 | 2.0 ± 1.6x | 3.1 ± 7.7x | 6.7 ± 1.6x | 11 ± 2.2x |
a
All dilutions were adjusted to 10% mannitol.
b
Data are mean values ± SD of two replicates for three independent experiments. At 0 h all plasmolysis values were 0x.
c
Values within the columns scored by the same letter were not statistically different at P = 0.05.
d
Undiluted concentrate adjusted to 0.2 μg of protein/ml in 10% mannitol.