FIG. 3.

(A) Induction of phage λ lysogens by high-pressure treatment (100 MPa, 15 min). Evolution of phage particle count [log(PFU/ml)] in untreated (▴) and high-pressure-treated (▪) cell suspensions of MG1655. (B) Fold increase in phage particle count (PFU/ml) 3 h after pressure treatment (100 MPa, grey bars) or UV treatment (0.1 kJ/m², dashed bars) in cell suspensions of the MG1655 wild type or ΔrecA or lexA1 mutant. Titers of phage for untreated cell suspensions were ca. 1.2 × 10³ PFU/ml for wild-type lysogens, 5 PFU/ml for ΔrecA lysogens, and 3.5 × 10³ PFU/ml for lexA1 lysogens.