TABLE 2.
Strains, phages, and plasmids used in this study
| Strain, phage, or plasmid | Genotype | Source or reference |
|---|---|---|
| E. coli strains | ||
| KM22 | Δ(recC ptr recB recD)::Plac-bet exo kan | 31 |
| ECL116 | F− ΔlacU169 endA hsdR thi | 2 |
| SME1074 | ECL116 λ SME106 | Laboratory collection |
| SME1216 | ECL116 λ SME103 | Laboratory collection |
| SME1851 | ECL116 λ SME104 | Laboratory collection |
| SME2416 | SME2417 zih-35::Tn10 | Laboratory collection |
| SME2417 | SME1216 Δ(recC ptr recB recD)::Plac-bet exo kan | This study |
| SME2495 | SME2417 ΔrhaSR::kan zih-35::Tn10 | This study |
| SME2496 | SME2416 ΔrhaSR::cat-sacB | This study |
| SME2508 | ECL116 λ SME114 recA::cat | Laboratory collection |
| SME2515 | ECL116 λ SME114 | Laboratory collection |
| SME2608 | SME1851 rhaS(wild type) recA::kan | Laboratory collection |
| SME2689 | SME1851 rhaS(D250A) zih-35::Tn10 recA::kan | This study |
| SME2691 | SME2515 rhaR(D276A) zih-35::Tn10 recA::kan | This study |
| SME2692 | SME2515 rhaR(D285A) zih-35::Tn10 recA::kan | This study |
| SME2693 | SME2515 rhaR(wild type) recA::kan | This study |
| SME2800 | SME1074 ΔrhaSR::kan zih-35::Tn10 | This study |
| SME2933 | SME2515 rhaR(E284A) zih-35::Tn10 recA::kan | This study |
| Phages | ||
| λ RS45 | bla′-lacZscatt+imm21ind+ | 40 |
| λ SME103 | λ RS45 φ(rhaB-lacZ)Δ110 | 11 |
| λ SME104 | λ RS45 φ(rhaB-lacZ)Δ84 | 11 |
| λ SME106 | λ RS45 φ(rhaS-lacZ)Δ216 | 11 |
| λ SME114 | λ RS45 φ(rhaS-lacZ)Δ92 | 17 |
| Plasmids | ||
| pGEX-2T σ70 | AprrpoD, wild type | 27 |
| pML148 to -169 | pGEX-2T, rpoD substitutions | 27 |
| pSE101 | pTZ18R AprrhaSR rhaBA′ | Laboratory collection |
| pSE172 | Tetr pALTER-1 rhaS D250A | Laboratory collection |
| pSE249 | pSE101 rhaS D250A | This study |
| pSE250 | pUC18 rhaSRT′, wild type | This study |
| pSE251 | pUC18 rhaSRT′ rhaR D276A | This study |
| pSE252 | pUC18 rhaSRT′ rhaR E284A | This study |
| pSE253 | pUC18 rhaSRT′ rhaR D285A | This study |
| pSE254 | pUC18 ΔrhaSR::cat-sac | This study |
| pUC18 | AprlacZα | 46 |