FIG. 1.

(A) Initial schematic map of CTn9343. The chromosomal DNA flanking the integrated element is shown by dotted lines. The putative excision-integration, transfer, oriT-mob, and rteA-rteB-satG-bexA regions are shown. The thin line between ORF 29 and the transfer region contains genes that are predicted to encode proteins contributing to resistance to bile (ORF 30), protection from restriction endonuclease cleavage (ORF 31), and partitioning of DNA into bacterial cells (ORF 32) (Table 3). The thin line between the transfer and excision-integration regions contains genes (ORFs 51 to 55) that encode proteins without significant identity to any protein in the GenBank database (Table 3). Arrows show the locations of representative ORFs and the direction of their transcription. (B) Relative locations of the PCR products used as probes to characterize CTn86. The results of PCR and hybridization to these probes are indicated beneath each probe (P, positive; N, negative). The thick bar between probes 5 and 6 indicates the ∼12-kb region sequenced in ETBF 86-5443-2-2. The BfPAI in ETBF 86-5443-2-2 is located between oriT and bfmC.