TABLE 3.
Putative ORFs in CTn9343 and flanking regions
| ORF (gene name)a | No. of amino acids | Putative protein | % Identity/ % similarity (range)b | E valuec | Source | Accession no. |
|---|---|---|---|---|---|---|
| 1 | 120 | No significant identity | ||||
| 2 | 462 | No significant identity | ||||
| 3 | 211 | No significant identity | ||||
| 4 | 187 | No significant identity | ||||
| 5 (int1) | 268 | Integrase | 63/81 (263) | 6e-95 | S. pneumoniae | AAK99251.1 |
| 6 | 342 | Type I R-M system (chain S) | 32/43 (343) | 3e-34 | S. pneumoniae | AAK99252.1 |
| 7 | 102 | Type I R-M system (chain S) | 33/54 (62) | 0.90 | S. pneumoniae | AAK99252.1 |
| 8 (tnpB) | 263 | IS21 transposase | 100/100 (263) | e-145 | B. fragilis | AF303352.2 |
| IstB (IS21) | 34/56 (239) | 1e-36 | E. coli | BVECIT | ||
| 9 (tnpA1) | 548 | IS21 transposase | 100/100 (548) | 0.00 | B. fragilis | AF303352.1 |
| IstA (IS21) | 27/43 (382) | 3e-20 | E. coli | BVECIS | ||
| 10 (int2) | 240 | Integrase | 91/92 (205) | e-104 | B. fragilis | AF303352.5 |
| Integrase (CTnDOT) | 65/78 (223) | 1e-82 | B. thetaiotaomicron | CAC47921.1 | ||
| 11 | 349 | Hypothetical protein | 31/51 (357) | 4e-42 | T. erythraeum | ZP00072163.1 |
| 12 | 221 | Hypothetical protein | 23/41 (186) | 5e-14 | T. erythraeum | ZP00072164.1 |
| 13 (rteB) | 440 | RteB | 98/98 (440) | 0.00 | B. fragilis | AF303352.3 |
| RteB (CTnDOT) | 85/93 (449) | 0.00 | B. thetaiotaomicron | AAA22921 | ||
| 14 (rteA) | 423 | RteA | 96/96 (423) | 0.00 | B. fragilis | AF303352.4 |
| RteA (CTnDOT) | 64/78 (337) | e-117 | B. thetaiotaomicron | A41860 | ||
| 15 (satG) | 205 | Acetyltransferase | 72/83 (205) | 2e-88 | B. thetaiotaomicron | AA077491.1 |
| Acetyltransferase | 65/79 (204) | 3e-79 | C. acetobutylicum | AAK78753.1 | ||
| 16 (bexA) | 235 | Putative cation efflux pump | 58/73 (229) | 1e-69 | B. thetaiotaomicron | AA077007.1 |
| 17 | 314 | Hypothetical protein | 55/69 (310) | 1e-89 | B. thetaiotaomicron | AA077572.1 |
| 18 (bfmA) | 180 | Mobilization protein A | 97/100 (180) | 9e-97 | B. fragilis | AAD05337.1 |
| 19 (bfmB) | 316 | Mobilization protein B | 97/98 (316) | e-180 | B. fragilis | AAD05338.1 |
| MobB | 26/42 (257) | 2e-15 | B. vulgatus | CAA63435 | ||
| 20 (bfmC) | 534 | Mobilization protein C | 98/99 (534) | 0.00 | B. fragilis | AAD05339.1 |
| TraG family protein | 23/45 (453) | 6e-30 | P. gingivalis | AAQ66535.1 | ||
| Type IV secretion, VirD4 | 21/40 (369) | 4e-04 | V. vulnificus | AA007601.1 | ||
| 21 | 117 | No significant identity | ||||
| 22 | 1221 | Hypothetical protein | 21/41 (551) | 1e-17 | H. sommus | ZP00122678.1 |
| 23 | 438 | Hypothetical protein | 29/56 (168) | 1e-17 | C. perfringes | NP561571.1 |
| 24 | 134 | Hypothetical protein | 32/53 (80) | 4e-04 | C. perfringes | NP561571.1 |
| 25 | 195 | RNA polymerase ECF-type sigma factor | 40/67 (170) | 2e-34 | B. thetaiotaomicron | AA077570.1 |
| 26 | 318 | Putative anti-sigma factor | 38/56 (316) | 3e-57 | B. thetaiotaomicron | AA077569.1 |
| 27 | 1127 | Putative outer membrane protein involved in nutrient binding | 32/50 (1,168) | e-161 | B. thetaiotaomicron | AA077568.1 |
| 28 | 545 | Putative outer membrane protein involved in nutrient binding | 28/44 (577) | 8e-44 | B. thetaiotaomicron | AA077302.1 |
| 29 (xyl) | 519 | Putative xylanase | 35/54 (495) | 7e-74 | B. thetaiotaomicron | AA078149.1 |
| 30 (bsh) | 359 | Choloylglycine hydrolase | 36/56 (341) | 1e-53 | B. melitensis | AAL51724.1 |
| Penicillin acylase | 31/51 (340) | 1e-35 | L. monocytogenes | CAC98525.1 | ||
| 31 | 326 | Methylase HpyI | 23/37 (217) | 0.05 | H. pylori | AAB07029.1 |
| 32 | 265 | Chromosome-partitioning ATPase | 36/57 (260) | 1e-41 | B. thetaiotaomicron | AAO45329.1 |
| 33 | 614 | No significant identity | ||||
| 34 | 527 | Cell surface protein | 23/39 (312) | 2e-04 | M. acetivorans | AAM07636.1 |
| 35 | 183 | Putative peptidase | 40/53 (189) | 9e-28 | B. thetaiotaomicron | AAO79101.1 |
| 36 (traN) | 384 | TraN-like protein (CTnDOT) | 33/57 (365) | 2e-55 | B. thetaiotaomicron | AA045314.1 |
| 37 | 149 | No significant identity | ||||
| 38 (traM) | 326 | Tra-M-like protein (CTnDOT) | 30/46 (325) | 4e-28 | B. thetaiotaomicron | AA045313.1 |
| 39 | 213 | Hypothetical protein | 40/60 (204) | 2e-35 | B. thetaiotaomicron | AA045312.1 |
| 40 | 257 | Hypothetical protein | 25/44 (264) | 2e-15 | B. thetaiotaomicron | AA045311.1 |
| 41 (traI) | 173 | Tra-I-like protein (CTnDOT) | 28/49 (182) | 2e-09 | B. thetaiotaomicron | AA045310.1 |
| 42 (traG) | 806 | Tra-G-like protein (CTnDOT) | 43/64 (803) | 0.00 | B. thetaiotaomicron | AA045309.1 |
| 43 | 86 | No significant identity | ||||
| 44 | 102 | Hypothetical protein | 37/61 (77) | 2e-09 | B. thetaiotaomicron | AA045307.1 |
| 45 | 265 | No significant identity | ||||
| 46 | 534 | Platelet binding protein GspB | 20/38 (349) | 4e-05 | S. gordii | AAL13053.1 |
| 47 | 320 | No significant identity | ||||
| 48 | 293 | No significant identity | ||||
| 49 | 687 | Hypothetical protein | 27/45 (211) | 1e-11 | B. thetaiotaomicron | AA077766.1 |
| 50 | 180 | Hypothetical protein | 37/57 (189) | 7e-30 | B. thetaiotaomicron | AA076173.1 |
| 51 | 140 | No significant identity | ||||
| 52 | 172 | No significant identity | ||||
| 53 | 115 | No significant identity | ||||
| 54 | 210 | No significant identity | ||||
| 55 | 242 | No significant identity | ||||
| 56 (prmN1) | 303 | PrmN1 | 37/51 (300) | 3e-44 | B. uniformis | AF238307 |
| 57 | 185 | No significant identity | ||||
| 58 | 369 | Hypothetical protein | 32/47 (370) | 1e-35 | B. thetaiotaomicron | AA079131.1 |
| Replication protein | 35/53 (128) | 3e-13 | Bacteriophage bIL286 | AF3236669.16 | ||
| DnaD domain protein | 29/49 (127) | 9e-09 | E. faecalis | AA081863.1 | ||
| 59 | 165 | Hypothetical protein | 32/57 (101) | 3e-13 | B. thetaiotaomicron | AA079131.1 |
| DnaD domain protein | 31/49 (99) | 3e-06 | E. faecalis | AA081863.1 | ||
| 60 | 105 | No significant identity | ||||
| 61 (tnpA2) | 124 | IS21 transposase (TnpA) | 44/65 (121) | 8e-23 | B. fragilis | AA303352.1 |
| 62 | 447 | Type I R-M system (chain S) | 33/50 (420) | 4e-51 | S. pneumoniae | AAK99252.1 |
| 63 | 148 | ABC-type sugar transport system | 25/49 (136) | 6e-06 | F. nucleatum | EAA24799.1 |
| ATPase component | ||||||
| Doc protein | 30/52 (88) | 7e-04 | S. oneidensi | AAN53454.1 | ||
| 64 | 338 | Hypothetical protein | 44/64 (337) | 1e-74 | Magnetococcus sp. | ZP 00044096.1 |
| 65 (hsdM) | 506 | Type I R-M system (M subunit) | 55/73 (498) | e-161 | S. pneumoniae | AAK74667.1 |
| 66 (hsdR) | 771 | Type I R-M system (R subunit) | 49/67 (774) | 0.00 | S. pneumoniae | AAK99254.1 |
a
Name based on sequence homology.
b
Identity and similarity are presented as percent amino acid identity and similarity between the CTn9343 ORF and the best hit. The range is the number of amino acids over which this identity and similarity exist. Identity and similarity were determined by BLAST (1).
c
E value of <0.05 was considered statistically significant (1).