Table 2.
Passage of brain homogenates from spontaneously ill Tg mice expressing mutant BVPrP(I109)
| Inoculum | Serial transmission to mice expressing homotypic PrP | Transmission to Tg(BVPrP,I109)3581 mice | Transmission to Tg(MoPrP)4053 mice | |||||
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| Tg line (mutation) | Age at onset of spontaneous illness (d) | Recipient line (mutation) | Mean incubation period ± SEM (d) | Signs of neurologic dysfunction (n/n0)a | Mean incubation period ± SEM (d) | Signs of neurologic dysfunction (n/n0)a | Mean incubation period ± SEM (d) | Signs of neurologic dysfunction (n/n0)a |
| Tg15972 (D178N) | 187 | Tg15972 (D178N) | 43 ± 6 | 8/8 | 46 ± 3 | 8/8 | 332 ± 20 | 7/7 |
| Tg15965 (D178N) | 183 | Tg15972 (D178N) | 62 ± 1 | 8/8 | 76 ± 4 | 8/8 | NDb | NDb |
| Tg15965 (D178N) | 203 | Tg15972 (D178N) | 60 ± 2 | 8/8 | 67 ± 4 | 7/7 | 421 ± 54c | 5/7 |
| Tg14210 (E200K) | 464 | Tg14210 (E200K) | 68 ± 1 | 8/8 | 50 ± 2 | 7/7 | NDb | NDb |
| Tg7253 (E200K) | 282 | Tg14210 (E200K) | 41 ± 1 | 8/8 | 36 ± 1 | 8/8 | 356 ± 10 | 5/5 |
| Tg4253 (E200K) | 194 | Tg14210 (E200K) | 51 ± 1 | 5/5 | 40 ± 0 | 7/7 | NDb | NDb |
| Tg7271 (E200K) | 131 | Tg14210 (E200K) | 46 ± 2 | 7/7 | 34 ± 1 | 8/8 | 304 ± 8 | 7/7 |
| Tg24600 (ΔGPI) | 349 | Tg24600 (ΔGPI) | 100 ± 5 | 6/6 | 47 ± 5 | 5/5 | NDb | NDb |
| Tg24600 (ΔGPI) | 408 | Tg24600 (ΔGPI) | 110 ± 9 | 8/8 | 42 ± 1 | 8/8 | NDb | NDb |
a
n, number of positive mice; n0, number of examined mice.
b
ND, not determined.
c
Incubation period calculated from five ill mice. Two mice remained healthy at 601 dpi.