TABLE 6.
Cytokine concentrations determined from supernatants of cocultured tracheal rings and PAMs at 6 and 15 hpi with PRRSV in vitro
| hpi and cytokine | Cytokine concn (pg/ml)a
|
|||
|---|---|---|---|---|
| Control | M. hyo | PRRSV | PRRSV and M. hyo | |
| 6 hpi | ||||
| IL-1β | 61.00 ± 50.19 | 104.33 ± 50.58 | 6.17 ± 10.68 | 37.00 ± 64.09 |
| IL-8 | 1.18 ± 0.12A | 1.57 ± 0.52B | 0.95 ± 0.21A | 1.28 ± 0.33A,B |
| IL-10 | 0.125 ± 0.0A | 11.69 ± 4.54B | 0.33 ± 0.36A | 18.36 ± 10.47B |
| TNF-α | 24.36 ± 3.79 | 29.08 ± 9.61 | 17.42 ± 3.56 | 26.17 ± 17.17 |
| 15 hpi | ||||
| IL-1β | 49.33 ± 18.09A | 396.83 ± 337.86B | 76.00 ± 34.37A | 359.33 ± 197.05A,B |
| IL-8 | 3.00 ± 0.82A | 13.05 ± 6.35B | 2.72 ± 0.59A | 10.79 ± 8.74A,B |
| IL-10 | 1.69 ± 1.56A | 18.56 ± 2.86B | 1.17 ± 1.80A | 18.45 ± 16.00B |
| TNF-α | 9.78 ± 5.01A,B | 14.50 ± 7.95B | 7.14 ± 6.13A | 12.28 ± 5.09B |
a
Cytokine concentrations were measured by a commercial ELISA. Values in rows with different letters (A, B, or C) are significantly different (P < 0.001). M. hyo, M. hyopneumoniae.