FIG. 4.
An ELISPOT assay performed using a CD8 T-cell line from subject 10, with overlapping 15-mer peptides, in pools of three, of the HPV 16 E6 and E7 protein, suggesting the presence of one antigenic epitope in the E6 46-70 region and another one in the E7 76-98 region. Each pool contained three 15-mer peptide which overlap by 10 central amino acids. For example, the first pool, which covered the E6 1-25 region, contained E6 1-15, E6 6-20, and E6 11- 25. A total of 2 × 105 cells from the CD8 T-cell line were plated per well.