TABLE 2.
NanH ELISA screening of rabbits housed in research facilities
| Sourcea | No. of rabbits negative/total no. tested (%) | No. of rabbits positive for the following/total no. tested (%):
|
|||
|---|---|---|---|---|---|
| IgMb | IgGb | IgM and IgGc | Total | ||
| Facility 1d | 3/3 (100) | 0/3 (0) | 0/3 (0) | 0/3 (0) | 0/3 (0) |
| Facility 2 | 15/15 (100) | 0/15 (0) | 0/15 (0) | 0/15 (0) | 0/15 (0) |
| Facility 3 | 6/11 (55) | 1/11 (9) | 4/11 (36) | 0/11 (0) | 5/11 (45) |
| Facility 4 | 3/21 (14) | 0/21 (0) | 6/21 (29) | 12/21 (57) | 18/21 (86) |
| Facility 5d | 9/15 (60) | 2/15 (13) | 3/15 (20) | 1/15 (7) | 6/15 (40) |
| Facility 6 | 0/4 (0) | 1/4 (25) | 2/4 (50) | 1/4 (25) | 4/4 (100) |
| Facility 7 | 3/7 (43) | 1/7 (14) | 3/7 (43) | 0/7 (0) | 4/7 (57) |
| Facility 8d | 3/10 (30) | 2/10 (20) | 4/10 (40) | 1/10 (10) | 7/10 (70) |
| Total | 42/86 (49) | 7/86 (8) | 22/86 (26) | 15/86 (17) | 44/86 (51) |
All research facilities except facilities 1 and 2 reported a recent history of pasteurellosis among rabbits in the same area.
Sample positive only for IgG or IgM but not both. To be considered positive for NanH antibody, samples exhibited an OD at 490 nm of 0.2 or above at a 1:16 dilution by the NanH IgG assay or an OD of 0.13 or above at a dilution of 1:8 by the NanH IgM assay. The NanH IgG ELISA detected nonisotype-specific IgG.
Samples positive for both IgM and IgG NanH antibodies.
Some samples from facilities 1, 5, and 8 were also tested by the whole-cell-lysate ELISA as described by Kawamoto et al. (29). Samples from facility 1 did not produce antibodies; however, both facilities 5 and 8 possessed rabbits positive for antibodies to whole-cell lysates.