Fig. 2.

NT-rfhSP-A is expressed as an insoluble protein but allows isolation of pure rfhSP-A. Purification samples were analysed by SDS-PAGE with subsequent Coomassie staining. (A) NT-rfhSP-A was expressed in inclusion bodies as indicated by the analysis of soluble and insoluble fraction of cell lysate after increasing numbers of washes (1, 2 and 3) of inclusion bodies. (B) Analysis of rfhSP-A purification samples after solubilisation and subsequent refolding (refolded), flow through of the nickel column (FT), washing of nickel column (Wash), purification of target fusion protein (NT-rfhSP-A), cleavage of target fusion protein (Cleaved) and purification of rfhSP-A from cleaved NT-rfhSP-A (rfhSP-A).