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Confirmation of trimeric rfhSP-A being functional in binding to mannan. (A) Various concentrations of rfhSP-A were applied to mannan coated plates in the presence of calcium or EDTA. Binding was detected using an antibody raised against nhSP-A. (B) Specificity of binding to mannan was confirmed by addition of 5 μg/ml of trimeric rfhSP-A in the presence of calcium with increasing amounts of soluble mannan. Displayed are mean ± SD of 3 experiments.
