FIG. 4.

Effects of PI-PLC treatment on IL-8 induction by P. aeruginosa LPS or S. aureus PGN in the uroepithelial cell lines T24 (A, B, and C) and 5637 (D, E, and F). (A and D) Cell surface expression of CD14 with or without PI-PLC treatment as determined by flow cytometry. Cells were treated with 0.5 U of PI-PLC/ml for 2 h and then analyzed by flow cytometry. Similar results were observed with treatment for as long as 14 h (data not shown). Dotted lines indicate staining with unrelated isotype-matched control antibodies. (B, C, E, and F) Induction of IL-8 after stimulation with LPS (B and E) or PGN (C and F) for 12 h in cells that were either left untreated or treated with PI-PLC. The concentration of IL-8 in the culture supernatant was determined by ELISA. Each experiment was performed in triplicate.