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. Author manuscript; available in PMC: 2016 Dec 12.
Published in final edited form as: Nat Protoc. 2016 Feb 4;11(3):413–428. doi: 10.1038/nprot.2016.012

Figure 2.

Figure 2

Examples of gel purification steps in the mNET-seq method. (a) RNA size purification of Pol II IP products from an 8% (wt/vol) urea gel (Step 55). The 35- to 100-nt-sized RNA should be cut from the unlabeled sample. At least one empty lane should separate the unlabeled and radiolabeled samples. (b) Gel analysis of amplified cDNA library in the mNET-seq method (Step 91). The library size should be 150–230 bp on the 6% (wt/vol) polyacrylamide gel (red arrow). (c) Gel analysis of purified cDNA library (Step 95). Make sure that there is no primer-dimer contamination.