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. Author manuscript; available in PMC: 2016 Dec 12.
Published in final edited form as: Nat Protoc. 2016 Feb 4;11(3):413–428. doi: 10.1038/nprot.2016.012

Figure 3.

Figure 3

Example of a mNET-seq profile for the SIK1 gene. The mNET-seq minus strand reads are shown on the SIK1 gene. Four different Pol II CTD antibodies (Table 2), CMA601 (Total), 8WG16 unphosphorylated CTD (Unph), CMA602 Ser2-P CTD (S2P) and CMA603 Ser5-P CTD (S5P), were used for the mNET-seq. The RNA from the chromatin fraction was also sequenced (ChrRNA-seq, bottom). mNET-seq detects the 3′ end of nascent RNA across the SIK1 gene and also downstream of the TES. CMA603 specifically immunoprecipitated 5′SS cleaved splicing intermediates.