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. Author manuscript; available in PMC: 2016 Dec 12.
Published in final edited form as: Nat Protoc. 2016 Feb 4;11(3):413–428. doi: 10.1038/nprot.2016.012
Reagents Volume (μl) per reaction Final concentration
Ultrapure water 8.5
PCR mix, 2× 25 1×
RNA PCR primer (RP1) 2
RNA PCR primer Index 2
cDNA library (from Step 84) 12.5
Mix total volume 50