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. Author manuscript; available in PMC: 2016 Dec 12.
Published in final edited form as: Nat Protoc. 2016 Feb 4;11(3):413–428. doi: 10.1038/nprot.2016.012
Reagents Volume (μl) per reaction Final concentration
Nuclease-free water 87
MNase buffer, 10× 10 1×
BSA, 100× 1 1×
MNase (2,000 gel units/μl) 2 40 gel unit/μl
Mix total volume 100