| Reagents | Volume (μl) per reaction | Final concentration |
|---|---|---|
| PNKT (buffer and Tween) | 160 | |
| PNK (20 units/μl) | 10 | 1 unit/μl |
| ATP, 10 mM | 30 | 1.5 mM |
| Mix total volume | 200 |
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