| Reagents | Volume (μl) per reaction | Final concentration |
|---|---|---|
| PNKT | 8.5 | |
| PNK (20 units/μl) | 0.5 | 1 unit/μl |
| 32P–γ-ATP, fresh | 1 | 1 μCi/μl |
| Mix total volume | 10 |
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