| Reagents | Volume (μl) per reaction | Final concentration |
|---|---|---|
| First-strand buffer, 5× | 2 | 1× |
| dNTP mix, 12.5 mM | 0.5 | 0.5 mM |
| DTT, 0.1 M | 1 | 8 μM |
| RNase inhibitor | 1 | 16 unit/μl |
| Superscript III reverse transcriptase | 1 | |
| Mix total volume | 5.5 |
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