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. 2016 Dec 12;11(12):e0167064. doi: 10.1371/journal.pone.0167064

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© 2016 Bhagirath et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A). K5⁺/K19^- or K5⁺/K19⁺ cell lines over-expressing vector or mRas/mp53/mPIK3CA oncogene combination were analyzed by Western Blotting. β-actin was used as a loading control. B). Representative images (magnification 20X) of colonies from K5⁺/K19^- and K5⁺/K19⁺ cells with vector or mRas/mp53/mPIK3CA oncogene combination assessed by anchorage independent growth assay. C) Quantification of colonies formed by different cells. Mean ± S.D. of a representative experiment done in triplicate is shown.