Table 2. Comparison of NCDO712 plasmids with earlier studies.
| Plasmids described in [26] | Plasmids analyzed in this study | ||||
|---|---|---|---|---|---|
| Plasmid | Size1 | Plasmid | Size | Plasmid copy number/mean coverage2 | Replication mode |
| pLP712 | 33 MDa, ~50 kb | pLP712 | 55 395 bp | 2 (423) | Theta |
| pSH713 | 1,8 MDa, ~3 kb | pSH71 | 2 062 bp | 3 (673) | RCR |
| pSH724 | 2,5 MDa, ~4 kb | pSH72 | 3 597 bp | 4 (921) | Theta |
| pSH735 | 5,2 MDa, ~8 kb | pSH73 | 8 663 bp | 3 (674) | Theta |
| pSH74 | 9 MDa, ~14 kb | pSH74 | 15 518 bp | 3 (697) | Theta |
| _ | _ | pNZ712 | 49 832 bp | 2 (471) | Theta |
1 Plasmid size in original publication is given in MDa (1MDa ds-DNA = 1.52 kb (https://tools.thermofisher.com).
2 Estimated on the bases sequence coverage in comparison to that of the chromosomal DNA. Coverage number is based on the analysis of 6 million sequence reads. The mean chromosomal DNA coverage in the same analysis was 198.
3 pSH71 in this study differs at 6 positions from the publically available sequence of pSH71 (NCBI accession number A09339; de Vos W.M., 1987): T712-deleted, T713-deleted, A731-deleted, G803A, T1234-inserted, C1414-deleted (the nucleotide before the position number indicates the sequence in the plasmid sequenced here, the description after the position number indicates the sequence in accession number A09339).
4 pSH72 differs by 3 bp from pND324 [70] (NCBI reference sequence: NC_008436.1): T1295G, G1384A and C3349-deleted. pSH72 is 99% identical to pND324
5 pSH73 is 100% identical to pAG6 (NCBI acession number: AB198069, GI: 70067197)