Skip to main content
. 2016 Dec 12;11(12):e0167965. doi: 10.1371/journal.pone.0167965

Fig 1. Construction and confirmation of Gpr55 KO mice.

Fig 1

(A) Schematic diagram of the mouse Gpr55 gene and the targeting strategy. CDS: coding sequence, 3′UTR: 3 end un-translated region, PGK Neo: a loxP-flanked Neo cassette. (B) Southern blotting analyses of ES-cell clones using the probes indicated in (A). (C) Gpr55 expression analyses by reverse transcriptase (RT) PCR analysis of cerebrum dissected from Gpr55 homozygous KO (lane 1–2), Gpr55 heterozygous (lane 3–4) and wild type (lane 5–6) mice.