Fig 3. Body weight gain, adiposity and effects of rimonabant.

(A) Body weight gain over 28 weeks during continuous high fat ‘café diet’ feeding in Gpr55 KO (n = 5) and corresponding WT (n = 7) mice. Statistical analysis by 2-Way ANOVA for repeated measures, with time and genotype as factors, indicated a significant effect of time (F_{28, 280} = 423.8, p<0.0001) but no difference between genotypes (F_{1, 10} = 0.1030, N.S.). (B) Correlation analysis of body weight (g) over relative body fat mass (g fat/ g body weight) in Gpr55 KO (n = 8) and corresponding WT (n = 8) mice at 2 months of age and in DIO Gpr55 KO (n = 5) and WT (n = 5) mice at 7 months of age. A linear regression model for adiposity with body weight and genotype as predictors to estimate relative adiposity indicated a non-significant trend (p ~0.10, N.S.) to increased adiposity in DIO Gpr55 KO vs. WT at 7 months of age. (C) Effects on body weight of daily dosing of rimonabant (20 μmol/kg p.o., once daily for 14 days) in Gpr55 KO (n = 5) and corresponding WT (n = 6) mice. Dashed line depicts the (100%) baseline level. Statistical analysis by 2-Way ANOVA for repeated measures, with time and genotype as factors, indicated a significant effect of time (F_{14, 126} = 162.6, p<0.0001) but no difference between genotypes (F_{1, 9} = 0.07729, N.S.).