Table 1. Information of the primers and corresponding candidate reference genes.
Gene | Accession No. | Correlation (r2) | Forward (F) and Reverse (R) Primers | Efficiency % | Amplicon length |
---|---|---|---|---|---|
gyrB | NZ_LAEN01000056.1 | 0.989 | F:CAACTTCATCGCCCATTAGG;R:GGGAGAAATGAACCCAGAAC | 88.2 | 191 |
recN | JN592546.1 | 0.981 | F:AGCTTGCTCTACCGCACAAT;R:CTGGCTTCTTGCACCTGAAT | 102.1 | 113 |
rpoB | NZ_AFFP02000004.1 | 0.994 | F:GCTTAATGCCGCTTCACCTA;R:AGCGTGTGGTGCAAGAAGAT | 99.1 | 131 |
infB | EU350938.1 | 0.999 | F:GCCAGTTGCTACCATTTTGG;R:AGCCTAGCACTTCCACAGGA | 97.3 | 155 |
pgi | JN592536.1 | 0.999 | F:GGAAAGGCTACACAGGCAAA;R:AACACAAGGGTGGTTTCTGG | 96.7 | 196 |
sodA | DQ005620.1 | 0.999 | F:TTAGCAGAAGTGCCAGCAGA;R:GCTTCCACAGAACCGAAATC | 92.7 | 155 |
atpD | AF326327.1 | 0.993 | F:TCCCACAAGATGCAGTACCA;R:CCCACTGGAACAGAAATTGG | 112.0 | 181 |
gyrA | NZ_AFFP02000003.1 | 0.993 | F:AGTGAGCGTAACGGCAAAGT;R:ATGTCCGATTCTTCGTCGTC | 98.7 | 218 |
16S rRNA | KF280244.1 | 0.997 | F:AGGCCTTCGGGTTGTAAAGT;R:CGGGGATTTCACATCTCACT | 99.2 | 201 |