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. 2016 Dec 12;11(12):e0167736. doi: 10.1371/journal.pone.0167736

Table 1. Information of the primers and corresponding candidate reference genes.

Gene Accession No. Correlation (r2) Forward (F) and Reverse (R) Primers Efficiency % Amplicon length
gyrB NZ_LAEN01000056.1 0.989 F:CAACTTCATCGCCCATTAGG;R:GGGAGAAATGAACCCAGAAC 88.2 191
recN JN592546.1 0.981 F:AGCTTGCTCTACCGCACAAT;R:CTGGCTTCTTGCACCTGAAT 102.1 113
rpoB NZ_AFFP02000004.1 0.994 F:GCTTAATGCCGCTTCACCTA;R:AGCGTGTGGTGCAAGAAGAT 99.1 131
infB EU350938.1 0.999 F:GCCAGTTGCTACCATTTTGG;R:AGCCTAGCACTTCCACAGGA 97.3 155
pgi JN592536.1 0.999 F:GGAAAGGCTACACAGGCAAA;R:AACACAAGGGTGGTTTCTGG 96.7 196
sodA DQ005620.1 0.999 F:TTAGCAGAAGTGCCAGCAGA;R:GCTTCCACAGAACCGAAATC 92.7 155
atpD AF326327.1 0.993 F:TCCCACAAGATGCAGTACCA;R:CCCACTGGAACAGAAATTGG 112.0 181
gyrA NZ_AFFP02000003.1 0.993 F:AGTGAGCGTAACGGCAAAGT;R:ATGTCCGATTCTTCGTCGTC 98.7 218
16S rRNA KF280244.1 0.997 F:AGGCCTTCGGGTTGTAAAGT;R:CGGGGATTTCACATCTCACT 99.2 201