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. 2016 Dec 12;11(12):e0168025. doi: 10.1371/journal.pone.0168025

Fig 5. GLI2 mediates the regulation of melanogenesis by primary cilia.

Fig 5

Melan-a cells were transfected with scrambled control (siNC) or GLI2 siRNA. After 24 h, the cells were treated with cytochalasin D (0.5 μM) or serum-free media. After 48 h, the proportion of ciliated cells was counted (A). Cellular melanin content was analyzed (B). GLI2, tyrosinase, and TRP1 were analyzed (C). B16F1 cells were transfected with siNC or GLI2 siRNA. After 1 day, the cells were treated with cytochalasin D (0.5 μM) or serum-free media for 2 days. Ciliated cells and cellular melanin content were analyzed (D, E). GLI2, tyrosinase, and TRP1 were analyzed (F). Data represent the mean ± SE of 3 experiments (* p < 0.05, ** p < 0.01).