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. 2004 Sep 1;18(17):2108–2119. doi: 10.1101/gad.1216004

Figure 4.

Figure 4.

Regulation of Rif1 by 53BP1 but not Chk2. (A) Immunoblotting analysis of the effect of 53BP1 siRNAs. HeLa cells were transfected twice with the indicated siRNAs and analyzed by immunoblotting 72 h after the first transfection for expression of the indicated proteins. Rif1 was detected with mouse 1060. (B) IR-induced Rif1 foci in cells treated with 53BP1 siRNA. Cells were exposed to 5 Gy 72 h after mock transfection or transfection with either control (GFP) or 53BP1 siRNAs. Cells were fixed 1 h after IR and processed as in Figure 2C. (C) IR-induced Rif1 foci in Chk2-/- HCT116 cells. HCT116 cells lacking functional Chk2 were treated with 5 Gy and processed for Rif1 and 53BP1 IF after 1 h as in Figure 2C.