Skip to main content
. 2004 Sep 1;18(17):2120–2133. doi: 10.1101/gad.312604

Figure 1.

Figure 1.

DAF-12 and DIN-1 interact. (A) Yeast two-hybrid interaction of DAF-12 (281–753) bait with DIN-1S constructs. Dilution series of the yeast strain L40ccu transformed with the indicated constructs, growth selected on media lacking leucine, tryptophan, (for bait [pBTM117] and prey [pACT] plasmids), histidine, and uracil (for two-hybrid interaction). (B) Yeast two-hybrid interaction of DIN-1S (1–567) bait with DAF-12 constructs. Asterisk indicates the dh115 derivative of the DAF-12 (1–617) rh61 mutant protein. Numbering is according to isoform DAF-12A1 except 1–737, isoform A3 (Antebi et al. 2000). (C, Top) Radiolabeled DIN-1S (61 kd) interacts in vitro with GST::DAF-12. (Bottom) Radiolabeled DAF-12 (84 kd) interacts with GST::DIN-1S. Fifty percent of the radiolabeled input used for incubations is shown.