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. 2016 Dec 12;12(12):e1006070. doi: 10.1371/journal.ppat.1006070

Fig 6. TcdA colocalizes with PACSIN2 during entry in Caco-2 cells.

Fig 6

(A) Immunofluorescence assays were performed as described in Fig 1B. At indicated time points, cells were fixed, stained for PACSIN2 and analyzed by confocal microscopy. Merged images show PACSIN2 in red, toxin in green and colocalization in yellow. Scale bars, 10 μm. The images shown are representative of multiple fields imaged from three independent experiments (B) Pearson’s correlation coefficient to assess the extent of colocalization between PACSIN2 and TcdA-546 at the indicated time points. Data represent mean and SD of 45 individual cells. (C) Mander’s coefficient to assess the fraction of TcdA-546 colocalizing with PACSIN2 and vice versa. A value of 1.0 indicates 100% overlap between the two colors. Data represent mean and SD of 45 individual cells. Cells were chosen at random for colocalizaton analyses.